Detection of influenza viruses through selective adsorption and detection of the M-protein antigen
Identifieur interne : 002307 ( Main/Exploration ); précédent : 002306; suivant : 002308Detection of influenza viruses through selective adsorption and detection of the M-protein antigen
Auteurs : Doris J. Bucher [États-Unis] ; Igor G. Kharitonenkov [Russie] ; M. Wajeed Khan [États-Unis] ; Arlette Palo [Russie] ; Denise Holloway [Russie] ; Adel Mikhail [Russie]Source :
- Journal of Immunological Methods [ 0022-1759 ] ; 1987.
English descriptors
- Teeft :
- Absorbance, Academic press, Adsorption, Adsorption time, Alkaline phosphatase, Allantoic fluid, Assay, Bead, Bovine, Bovine serum albumin, Bucher, Carbonate, Carbonate buffer, Carbonate coating buffer, Crossreactive epitope, Diethanolamine buffer, Elisa, Elisa assay, Higher levels, Hydrophobic properties, Hyperimmune, Hyperimmune antiserum, Hyperimmune sera, Influenza, Influenza virus, Influenza virus preparations, Influenza viruses, Kilbourne, Levy place, Lipid bilayer, Methods section, Microtiter plates, Model system, Optimized protocol, Parainfluenza virus type, Parent strain, Phosphate substrate, Polystyrene, Polystyrene bead, Polystyrene beads, Polystyrene surface, Practical virology, Rabbit serum, Recombinant strain, Research methods, Rhabdovirus group, Room temperature, Sarkosyl, Selective adsorption, Selective adsorption technique, Sendai, Sendai virus, Serum albumin, Sigma chemical, Sinai school, Swine influenza, Test system, Throat wash specimens, Veal infusion broth, Veterinary diseases, Viral, Viral agents, Viral particles, Virology, Virus, Virus preparations.
Abstract
Abstract: A model system has been developed which permits rapid detection of influenza viruses through targeting of the M (membrane or matrix)-protein; a type-specific antigen, in an enzyme-linked immunosorbent assay system. This technique exploits the hydrophobic properties of M-protein; the M-protein is selectively and rapidly adsorbed to polysterene surfaces even in the presence of a 5000-fold excess of bovine serum albumin. Hyperimmune antiserum prepared to purified M-protein is used as the detecting reagent. All type A influenza viruses could be detected by this technique, type B influenza viruses reacted to a slight extent and Sendai virus (parainfluenza virus, type 1) did not react. Virus could be detected to levels as low as 3 ng. Purification of M-protein and preparation of hyperimmune sera from other related virus groups, such as type B influenza viruses, paramyxoviruses and rhabdoviruses should permit detection of these agents by a similar technique.
Url:
DOI: 10.1016/0022-1759(87)90370-X
Affiliations:
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Levy Place, New York, NY 10029, U.S.A.Ivanovsky Institute of Virology, Gameleja Street 16, Moscow 123098</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">96</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="77">77</biblScope><biblScope unit="page" to="85">85</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorbance</term><term>Academic press</term><term>Adsorption</term><term>Adsorption time</term><term>Alkaline phosphatase</term><term>Allantoic fluid</term><term>Assay</term><term>Bead</term><term>Bovine</term><term>Bovine serum albumin</term><term>Bucher</term><term>Carbonate</term><term>Carbonate buffer</term><term>Carbonate coating buffer</term><term>Crossreactive epitope</term><term>Diethanolamine buffer</term><term>Elisa</term><term>Elisa assay</term><term>Higher levels</term><term>Hydrophobic properties</term><term>Hyperimmune</term><term>Hyperimmune antiserum</term><term>Hyperimmune sera</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus preparations</term><term>Influenza viruses</term><term>Kilbourne</term><term>Levy place</term><term>Lipid bilayer</term><term>Methods section</term><term>Microtiter plates</term><term>Model system</term><term>Optimized protocol</term><term>Parainfluenza virus type</term><term>Parent strain</term><term>Phosphate substrate</term><term>Polystyrene</term><term>Polystyrene bead</term><term>Polystyrene beads</term><term>Polystyrene surface</term><term>Practical virology</term><term>Rabbit serum</term><term>Recombinant strain</term><term>Research methods</term><term>Rhabdovirus group</term><term>Room temperature</term><term>Sarkosyl</term><term>Selective adsorption</term><term>Selective adsorption technique</term><term>Sendai</term><term>Sendai virus</term><term>Serum albumin</term><term>Sigma chemical</term><term>Sinai school</term><term>Swine influenza</term><term>Test system</term><term>Throat wash specimens</term><term>Veal infusion broth</term><term>Veterinary diseases</term><term>Viral</term><term>Viral agents</term><term>Viral particles</term><term>Virology</term><term>Virus</term><term>Virus preparations</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A model system has been developed which permits rapid detection of influenza viruses through targeting of the M (membrane or matrix)-protein; a type-specific antigen, in an enzyme-linked immunosorbent assay system. This technique exploits the hydrophobic properties of M-protein; the M-protein is selectively and rapidly adsorbed to polysterene surfaces even in the presence of a 5000-fold excess of bovine serum albumin. Hyperimmune antiserum prepared to purified M-protein is used as the detecting reagent. All type A influenza viruses could be detected by this technique, type B influenza viruses reacted to a slight extent and Sendai virus (parainfluenza virus, type 1) did not react. Virus could be detected to levels as low as 3 ng. Purification of M-protein and preparation of hyperimmune sera from other related virus groups, such as type B influenza viruses, paramyxoviruses and rhabdoviruses should permit detection of these agents by a similar technique.</div></front></TEI><affiliations><list><country><li>Russie</li><li>États-Unis</li></country><region><li>District fédéral central</li><li>État de New York</li></region><settlement><li>Moscou</li></settlement></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Bucher, Doris J" sort="Bucher, Doris J" uniqKey="Bucher D" first="Doris J." last="Bucher">Doris J. Bucher</name></region><name sortKey="Wajeed Khan, M" sort="Wajeed Khan, M" uniqKey="Wajeed Khan M" first="M." last="Wajeed Khan">M. Wajeed Khan</name></country><country name="Russie"><region name="District fédéral central"><name sortKey="Kharitonenkov, Igor G" sort="Kharitonenkov, Igor G" uniqKey="Kharitonenkov I" first="Igor G." last="Kharitonenkov">Igor G. Kharitonenkov</name></region><name sortKey="Holloway, Denise" sort="Holloway, Denise" uniqKey="Holloway D" first="Denise" last="Holloway">Denise Holloway</name><name sortKey="Mikhail, Adel" sort="Mikhail, Adel" uniqKey="Mikhail A" first="Adel" last="Mikhail">Adel Mikhail</name><name sortKey="Palo, Arlette" sort="Palo, Arlette" uniqKey="Palo A" first="Arlette" last="Palo">Arlette Palo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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